Review



human synapsin1 promoter hsyn1p  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc human synapsin1 promoter hsyn1p
    MAPK and calcium signaling is reduced in Taok2 cKO neurons (A–D) Luciferase assay using EGR1p (A), SARE- (B), FOSBp- (C), and CRE- (D) sensor-detected MAPK (A–C) and calcium (B, D)-dependent signaling activity in primary cortical neurons. Neurons were treated with AMPA (1 μM), BDNF (10 ng/mL), or bicuculline (BIC, 1 μM) on day in vitro 12 (DIV12) for 4 h. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant; Student’s t test (two-sided). (E) Imaging of control and Taok2 cKO primary cortical neurons infected with AVVs expressing the <t>synapsin1-driven</t> GCaMP6f calcium sensor. Images were taken before and after stimulating cells with 25 mM KCl. (F) Quantification of cellular GCaMP6f responses to 25 mM KCl. Intensities from responding cells were normalized to the background and pooled for analysis. Gray arrowheads on the x axis represent the time points at which images were taken before and after stimulation as shown in (E). The line represents the mean, and the shaded area represents the SEM; n = 30 responding cells from two independent cultures. ∗∗∗ p < 0.001 and mixed ANOVA. (G) Western blots indicate reduced phosphorylated Mek1/2 (p-Mek1/2) and Erk1/2 (p-Erk1/2) in primary mouse cortical neurons. Neurons were treated with AMPA (1 μM) on DIV12 for 4 h. (H and I) Quantification of western blot data shown in (G). Data of p-Erk1/2 are relative to total Erk1/2 (H), and data of p-Mek1/2 are relative to total Mek1/2 (I). Values are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; Wilcoxon rank-sum test (two-sided).
    Human Synapsin1 Promoter Hsyn1p, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human synapsin1 promoter hsyn1p/product/Addgene inc
    Average 93 stars, based on 6 article reviews
    human synapsin1 promoter hsyn1p - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "TAOK2 controls synaptic plasticity and anxiety via ERK and calcium signaling"

    Article Title: TAOK2 controls synaptic plasticity and anxiety via ERK and calcium signaling

    Journal: iScience

    doi: 10.1016/j.isci.2025.113712

    MAPK and calcium signaling is reduced in Taok2 cKO neurons (A–D) Luciferase assay using EGR1p (A), SARE- (B), FOSBp- (C), and CRE- (D) sensor-detected MAPK (A–C) and calcium (B, D)-dependent signaling activity in primary cortical neurons. Neurons were treated with AMPA (1 μM), BDNF (10 ng/mL), or bicuculline (BIC, 1 μM) on day in vitro 12 (DIV12) for 4 h. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant; Student’s t test (two-sided). (E) Imaging of control and Taok2 cKO primary cortical neurons infected with AVVs expressing the synapsin1-driven GCaMP6f calcium sensor. Images were taken before and after stimulating cells with 25 mM KCl. (F) Quantification of cellular GCaMP6f responses to 25 mM KCl. Intensities from responding cells were normalized to the background and pooled for analysis. Gray arrowheads on the x axis represent the time points at which images were taken before and after stimulation as shown in (E). The line represents the mean, and the shaded area represents the SEM; n = 30 responding cells from two independent cultures. ∗∗∗ p < 0.001 and mixed ANOVA. (G) Western blots indicate reduced phosphorylated Mek1/2 (p-Mek1/2) and Erk1/2 (p-Erk1/2) in primary mouse cortical neurons. Neurons were treated with AMPA (1 μM) on DIV12 for 4 h. (H and I) Quantification of western blot data shown in (G). Data of p-Erk1/2 are relative to total Erk1/2 (H), and data of p-Mek1/2 are relative to total Mek1/2 (I). Values are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; Wilcoxon rank-sum test (two-sided).
    Figure Legend Snippet: MAPK and calcium signaling is reduced in Taok2 cKO neurons (A–D) Luciferase assay using EGR1p (A), SARE- (B), FOSBp- (C), and CRE- (D) sensor-detected MAPK (A–C) and calcium (B, D)-dependent signaling activity in primary cortical neurons. Neurons were treated with AMPA (1 μM), BDNF (10 ng/mL), or bicuculline (BIC, 1 μM) on day in vitro 12 (DIV12) for 4 h. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant; Student’s t test (two-sided). (E) Imaging of control and Taok2 cKO primary cortical neurons infected with AVVs expressing the synapsin1-driven GCaMP6f calcium sensor. Images were taken before and after stimulating cells with 25 mM KCl. (F) Quantification of cellular GCaMP6f responses to 25 mM KCl. Intensities from responding cells were normalized to the background and pooled for analysis. Gray arrowheads on the x axis represent the time points at which images were taken before and after stimulation as shown in (E). The line represents the mean, and the shaded area represents the SEM; n = 30 responding cells from two independent cultures. ∗∗∗ p < 0.001 and mixed ANOVA. (G) Western blots indicate reduced phosphorylated Mek1/2 (p-Mek1/2) and Erk1/2 (p-Erk1/2) in primary mouse cortical neurons. Neurons were treated with AMPA (1 μM) on DIV12 for 4 h. (H and I) Quantification of western blot data shown in (G). Data of p-Erk1/2 are relative to total Erk1/2 (H), and data of p-Mek1/2 are relative to total Mek1/2 (I). Values are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; Wilcoxon rank-sum test (two-sided).

    Techniques Used: Luciferase, Activity Assay, In Vitro, Imaging, Control, Infection, Expressing, Western Blot



    Similar Products

    human synapsin1 promoter hsyn1p  (Addgene inc)
    93
    Addgene inc human synapsin1 promoter hsyn1p
    MAPK and calcium signaling is reduced in Taok2 cKO neurons (A–D) Luciferase assay using EGR1p (A), SARE- (B), FOSBp- (C), and CRE- (D) sensor-detected MAPK (A–C) and calcium (B, D)-dependent signaling activity in primary cortical neurons. Neurons were treated with AMPA (1 μM), BDNF (10 ng/mL), or bicuculline (BIC, 1 μM) on day in vitro 12 (DIV12) for 4 h. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant; Student’s t test (two-sided). (E) Imaging of control and Taok2 cKO primary cortical neurons infected with AVVs expressing the <t>synapsin1-driven</t> GCaMP6f calcium sensor. Images were taken before and after stimulating cells with 25 mM KCl. (F) Quantification of cellular GCaMP6f responses to 25 mM KCl. Intensities from responding cells were normalized to the background and pooled for analysis. Gray arrowheads on the x axis represent the time points at which images were taken before and after stimulation as shown in (E). The line represents the mean, and the shaded area represents the SEM; n = 30 responding cells from two independent cultures. ∗∗∗ p < 0.001 and mixed ANOVA. (G) Western blots indicate reduced phosphorylated Mek1/2 (p-Mek1/2) and Erk1/2 (p-Erk1/2) in primary mouse cortical neurons. Neurons were treated with AMPA (1 μM) on DIV12 for 4 h. (H and I) Quantification of western blot data shown in (G). Data of p-Erk1/2 are relative to total Erk1/2 (H), and data of p-Mek1/2 are relative to total Mek1/2 (I). Values are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; Wilcoxon rank-sum test (two-sided).
    Human Synapsin1 Promoter Hsyn1p, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human synapsin1 promoter hsyn1p/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    human synapsin1 promoter hsyn1p - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    MAPK and calcium signaling is reduced in Taok2 cKO neurons (A–D) Luciferase assay using EGR1p (A), SARE- (B), FOSBp- (C), and CRE- (D) sensor-detected MAPK (A–C) and calcium (B, D)-dependent signaling activity in primary cortical neurons. Neurons were treated with AMPA (1 μM), BDNF (10 ng/mL), or bicuculline (BIC, 1 μM) on day in vitro 12 (DIV12) for 4 h. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant; Student’s t test (two-sided). (E) Imaging of control and Taok2 cKO primary cortical neurons infected with AVVs expressing the synapsin1-driven GCaMP6f calcium sensor. Images were taken before and after stimulating cells with 25 mM KCl. (F) Quantification of cellular GCaMP6f responses to 25 mM KCl. Intensities from responding cells were normalized to the background and pooled for analysis. Gray arrowheads on the x axis represent the time points at which images were taken before and after stimulation as shown in (E). The line represents the mean, and the shaded area represents the SEM; n = 30 responding cells from two independent cultures. ∗∗∗ p < 0.001 and mixed ANOVA. (G) Western blots indicate reduced phosphorylated Mek1/2 (p-Mek1/2) and Erk1/2 (p-Erk1/2) in primary mouse cortical neurons. Neurons were treated with AMPA (1 μM) on DIV12 for 4 h. (H and I) Quantification of western blot data shown in (G). Data of p-Erk1/2 are relative to total Erk1/2 (H), and data of p-Mek1/2 are relative to total Mek1/2 (I). Values are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; Wilcoxon rank-sum test (two-sided).

    Journal: iScience

    Article Title: TAOK2 controls synaptic plasticity and anxiety via ERK and calcium signaling

    doi: 10.1016/j.isci.2025.113712

    Figure Lengend Snippet: MAPK and calcium signaling is reduced in Taok2 cKO neurons (A–D) Luciferase assay using EGR1p (A), SARE- (B), FOSBp- (C), and CRE- (D) sensor-detected MAPK (A–C) and calcium (B, D)-dependent signaling activity in primary cortical neurons. Neurons were treated with AMPA (1 μM), BDNF (10 ng/mL), or bicuculline (BIC, 1 μM) on day in vitro 12 (DIV12) for 4 h. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; n.s., not significant; Student’s t test (two-sided). (E) Imaging of control and Taok2 cKO primary cortical neurons infected with AVVs expressing the synapsin1-driven GCaMP6f calcium sensor. Images were taken before and after stimulating cells with 25 mM KCl. (F) Quantification of cellular GCaMP6f responses to 25 mM KCl. Intensities from responding cells were normalized to the background and pooled for analysis. Gray arrowheads on the x axis represent the time points at which images were taken before and after stimulation as shown in (E). The line represents the mean, and the shaded area represents the SEM; n = 30 responding cells from two independent cultures. ∗∗∗ p < 0.001 and mixed ANOVA. (G) Western blots indicate reduced phosphorylated Mek1/2 (p-Mek1/2) and Erk1/2 (p-Erk1/2) in primary mouse cortical neurons. Neurons were treated with AMPA (1 μM) on DIV12 for 4 h. (H and I) Quantification of western blot data shown in (G). Data of p-Erk1/2 are relative to total Erk1/2 (H), and data of p-Mek1/2 are relative to total Mek1/2 (I). Values are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; Wilcoxon rank-sum test (two-sided).

    Article Snippet: On DIV6, half the medium was changed and the neurons were infected with AAVs for expression of mRuby2-P2A-GCaMP6f under the control of the human synapsin1 promoter (hSyn1p) (Addgene #50943) at an MOI of 1000.

    Techniques: Luciferase, Activity Assay, In Vitro, Imaging, Control, Infection, Expressing, Western Blot